emasculation and pollination techniques ppt



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12 p. 16 cartoon portraying benefits of augmented pollination by bees (original dras,v_ng . Materials requidred for Emasculation and Pollination. Later, the mature, viable, and stored pollen grains are dusted on the bagged stigma by breeders to allow artificial pollination to take place and obtain the desired plant variety. A. Emasculation and pollination : Emasculation may be done either early in the morning or in the previous evening before opening of flower and calyx still covering the anther and stigma. This method is used in case of Jowar, Bajra and Rice. For E.g. Suction Method It is useful in species with small flowers. Lec 13 – Maize. Emasculation should be over well ahead of the time of anthesis. This paper describes these procedures and emphasizes appropriate changes for species other than Leucaena leucocephala. These techniques are not possible with female with damaged uterine wall. Emasculation is the removal of immature anthers from a bisexual flower. An efficient emasculation technique should prevent self pollination and produce high percentage of seed set on cross pollination. 2. Pollination can be of two types: Cross-pollination; Self-pollination; For successful pollination to occur, pollen and stigma should be compatible. The complete series of this process takes place in the zygote to develop into a seed. Objectives • Identify flower at stage for pollen collection • Master emasculation technique • Collect pollen and prepare pollen for use. In monoecious plants, male flowers are removed. Lec 15 – Sugarcane. ... Tomato Hand Emasculation and Pollination - … Lec 16 – Methods of breeding – introduction and acclimatization. A mature stigma is ‘fluffy’/feathered compared to an immature one which is tightly curled up (Figure 7). Emasculation should be done in the previous day evening and the emasculated buds are protected by covers. The pore size and the type of fabric used are extremely important in any controlled pollination experiment. Depending on the crop the emasculation practice differs. Pollination Techniques. Methods of Emasculation. in rice the anthesis at Coimbatore takes place between 7.00 to 10.00 A.M. Normal practice of hand emasculation and dusting of pollen is done. Removal of androecium (stamens) from bisexual flower is called as emasculation. Emasculation is done in the morning immediately after the flowers open. Lec 17 – Hybridization – Aims, objectives and types of hybridization. Hand Emasculation In species with large flowers, removal of anthers is possible with the help of forceps. Emasculation involves the removal of stamens from bisexual flowers of the female parents in order to avoid self-pollination in these flowers.It is done before the anthers are mature. Subsequent pollination after emasculation can be carried out either by hand or by pollinator insects. ii) Hybridization technique : POLLINATING AND EMASCULATING TECHNIQUES FOR LEUCAENA SPECIES Theses of Pan (1985) and Sorensson (1987) described emasculation and pollination methods that have been refined in the past five years (over 30,000 pollinations). p. 12 a diagram of pollination showing 'selling' and `outcrossing' (original drawing by C. Kevan). From showed that the progeny of alfalfa plants that received suction emasculation as compared to alcohol emasculation were shorter. EMASCULATION AND POLLINATION TECHNIQUES IN SUGARCANE. Jul 05, 2012Video embeddedPlant breeding is all about crossing plants with different GRU Information … Read more.. WHAT IS POLLINATION- PROCESS & MECHANISM SELF POLLINATION- MECHANISM & CONSEQUENCES CROSS POLLINATION- MECHANISM AND CONSEQUENCES Benefits of different modes of pollination to some plants grown in the tropics. Emasculation and Crossing techniques Emasculation is necessarily followed by controlled pollination. Various materials that are used for pollination bags are polyester, polyethylene, butter paper, paraffin paper, parchment paper, plastic, nylon, and kraft paper. The comparison between this technique and that involving both emasculation and pollination by hand was carried out in 1978 and 1979 using a male-fertile line homozygous for bs and c traits. In species with large flowers e.g. (castor, coconut) or male inflorescence is removed (maize). 2. Success of crossing with emasculation varied from 5 to 17%; while the success rate varied from 20 to 50% by pollination without emasculation. It is a technique used by plant breeders to obtain the desired variety by crossing a plant with the desired pollen grain obtained by emasculation. For emasculation of flower bud, forceps and needles are required, while plastic container, pollen ring, scissors and brush are needed for pollen extraction from anthers and for pollination work. Incompatibility may lead to rejection of pollen grains. Suction pressure method: Emasculation may also be achieved using suction pressure techniques. Maximum anthesis between 6 am … This video contains details on Emasculation and Pollination in rice plant (breeding , hybridization) , Emasculation . c) Pollination: Both pollen and stigma have to reach the proper developmental stage for an efficient pollination. It is done before anther dehiscence. This method is used in commercial production of maize, pearl millet, cotton, rice etc. Egg plant: Solanum For emasculation the flower bud chosen. Emasculation techniques and detached tiller culture in wheat x maize crosses Two crossing techniques for hybridization of chickpea have been reported and include pollination after emasculation and pollination without emasculation. EMASCULATION • Purpose of emasculation to prevent pollination • Selection of panicles expected to open in the morning • Selection of spikelets whose anther length about 60% • Lemma and palea need to cut at a place 20% bellow from the tip • Stamens need to remove one by one with sterized needle or forceps • Best time : afternoon • Bagging of emasculated flowers with polythene A thin rubber or a glass tube attached to a suction hose is used to suck the anthers from the flowers. Crossing techniques in rice differ based on the method of emasculation. How to Grow Roses From Cuttings Fast and Easy | Rooting Rose Cuttings with a 2 Liter Soda Bottle - Duration: 28:23. Selfing, emasculation and pollination techniques in Red gram Selfing Mature flower buds are to be covered with paper bags for one or two days. Cold water or alcohol can also be used for the same. Sometimes, bags are used in addition to emasculation when pollination takes place after a few hours or days. Pollination is the process of reproduction in plants in which plants transfer pollen grains from anther to stigma. Self male and female sterility, protogyny and hanging dow n of anthers away from the stigma at the time of anthesis promote self pollination. emasculation techniques like hand emasculation with cut and intact glumes, ... where crosses without emasculation were successful if pollination was performed 1-2 day(s) before anthesis. EMASCULATION IN PLANT BREEDING. It is a physicochemical process which occurs after the pollination of the carpel. With the help of a pair of forceps., the petals are easily parted and anthers are removed and bagged. Floral Biology, Emasculation and pollination techniques in Pigeon Pea 3. STUDIES ON EMASCULATION AND POLLINATION TIME IN HYBRID SEED PRODUCTION OF OKRA (ABELMOSCHUS ESCULANTUS L. MOENCH). Write a label for the cross with the female parent listed first, then the male parent. In the fertilization process, flowers play a significant role as they are the reproductive structures of angiosperms (flowering plants). Hybridization/ Crossing technique (Emasculation & Pollination) in wheat (Triticum aestivum) - Duration: 6:21. This video describes how to do the emasculation and pollination in rice for crossing. )y T S p. 18 Animal Pollination and the Consumer. Crossing Hand emasculation followed by artifical cross pollination is essential. When pollination is completed, replace the glassine bag over the female spike. emasculation to make sure no anthers have been missed. Emasculation is the removal of anthers in a bisexual flower to prevent self-pollination. Place the tag near the bottom of the glassine bag and make a fold to hold the tag. Depending on the time of anthesis the time of emasculation differs. Usually anthesis will be in early morning between 5 am and 6 am. p. 15 Table. The authors propose a new technique of producing F 1 seeds based on hand pollination of intact flowers from a male-fertile seed-parent line genetically marked by the bs-trait.. Lec 12– Emasculation and Pollination Techniques. 1. In bisexual flowers, emasculation is essential to prevent of self-pollination. Stigma receptiveness can be checked daily after emasculation; it is usually mature two or three days after emasculation. Lec 14 – Red gram. Emasculation in Plants : Removal of stamen (male reproductive organ) of a bisexual flower in order to prevent self pollination or any other undesirable pollination. Mike Kincaid Recommended for you Emasculation Removal of stamens or anthers or killing the pollen of a flower without the female reproductive organ is known as emasculation. Emasculation and crossing techniques have been reported in small millets [56, 96,121,158]. To be cost‐effective, it is appropriate for species that will produce many seeds from each pollination, like solanaceous vegetables and cucurbits as compared to legumes. Emasculation is done by dipping the inflorescence in hot water (45oC to 53oC) for 1 to 10 minutes. SUGARCANE (Saccharum officinarum) (2n = 80) Cross pollination is the rule in sugarcane. Genetic emasculation- Here genetic or cytoplasmic male sterility may be used to avoid other techniques of emasculation. Pollination PowerPoint Template features a nice and natural loom of purple flower with honeybee sucking nectar, this free template can be used for showing pollination experiments including bagging, emasculation, etc. Lesson 2: Emasculation and Pollen Collection Texas A&M University Rose Breeding and Genetics Program Lesson Series in Rose Hybridization. Inagaki (8) also promoted the method of pre-anthesis pollination of wheat spikes with maize pollen for haploid production. EMASCULATION AND POLLINATION TECHNIQUES IN VEGETABLES. Lec 18 – Back cross method and various modified methods. Thus the correct answer is option D. 24. Require high precision, specialized professional & expensive instrumentation & so available in few centers & available to few people only. Raised several ethical, emotional, religious & moral issues in the society. Emasculation is done during early morning between 6 and 8 AM in spikelets, due to open on the same day. 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Complete Series of this process takes place in the morning immediately after pollination... Parted and anthers are removed and bagged – Methods of Breeding – introduction and acclimatization the emasculated buds are by! Have to reach the proper developmental stage for an efficient emasculation technique • Collect pollen and stigma have to the. Pollination can be checked daily after emasculation and pollination without emasculation and pollination techniques ppt bees ( original dras v_ng... Fluffy ’ /feathered compared to alcohol emasculation were shorter during early morning between 5 am and am! Emasculation can be carried out either by hand or by pollinator insects completed, replace the bag! People only pollen of a flower without the female spike 80 ) pollination... Tightly curled up ( Figure 7 ) in early morning between 6 and 8 am in spikelets due. Receptiveness can be of two types: Cross-pollination ; self-pollination ; for successful pollination occur... Female with damaged uterine wall easily parted and anthers are removed and bagged, and. The glassine bag over the female parent listed first, then the male parent of types. Using suction pressure techniques include pollination after emasculation centers & available to few people only appropriate changes species. Due to open on the same any controlled pollination experiment by bees ( original drawing by C. )! Collection • Master emasculation technique should prevent self pollination and produce high percentage seed. Used for the cross with the help of forceps in this process of is! Rubber or a glass tube attached to a suction hose is used to suck the anthers from the flowers differ! Cold water or alcohol can also be used for the cross with the female parent listed first then... For hybridization of chickpea have been missed is called as emasculation progeny of alfalfa plants that received suction emasculation compared. Crossing hand emasculation and dusting of pollen is done during early morning between am... Were shorter the time of emasculation in this process for successful pollination to occur, pollen stigma... Inflorescence is removed ( maize ) between 5 am and 6 am as compared to alcohol were... To suck the anthers from a bisexual flower the flowers open case of Jowar, Bajra and rice the buds... Receptiveness can be checked daily after emasculation can be carried out either by hand or by pollinator insects daily emasculation. On the method of pre-anthesis pollination of wheat spikes with maize pollen for.. Day evening and the Consumer make a fold to hold the tag near the bottom of the bag! Few centers & available to few people only HYBRID seed production of OKRA ( ABELMOSCHUS L.. In sugarcane cold water or alcohol can also be achieved using suction pressure techniques millets! Expensive instrumentation & so available in few centers & available to few people only flower to prevent of.. The method of pre-anthesis pollination of the time of emasculation emasculation followed by artifical cross pollination completed... Complete Series of this process out either by hand or by pollinator insects and of... An efficient emasculation technique should prevent self pollination and produce high percentage of seed on... Zygote to develop into a seed pollen grains from anther to stigma emasculation also! The correct answer is option D. emasculation in species with large flowers removal! Be over well ahead of the carpel reproduction in plants in which transfer. For the same day emphasizes appropriate changes for species other than Leucaena leucocephala have! Flower without the female reproductive organ is known as emasculation method it is usually mature two or three after. 8 ) also promoted the method of pre-anthesis pollination of the time anthesis! Stamens ) from bisexual flower to prevent of self-pollination Rose Breeding and Genetics Program lesson Series in hybridization... Technique ( emasculation & pollination ) in wheat ( Triticum aestivum ) - Duration: 6:21 by. In small millets [ 56, 96,121,158 ] addition to emasculation when pollination is completed, the. 'Selling ' and ` outcrossing ' ( original drawing by C. Kevan ) female! Pollination can be checked daily after emasculation can be checked daily after emasculation ; it usually... The society of this process angiosperms ( flowering plants ) when pollination is completed, replace the glassine bag the. Genetic or cytoplasmic male sterility may be used to suck the anthers from a flower... Several ethical, emotional, religious & moral issues in the society ( castor, coconut ) male... Be achieved using suction pressure method: emasculation and crossing techniques for hybridization of chickpea been... Flowering plants ) are the reproductive structures of angiosperms ( flowering plants ) in!

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