This is because liver contains the enzyme catalase, which breaks down the hydrogen peroxide into oxygen gas and water. LIVER CATALASE I. Who is the longest reigning WWE Champion of all time? If the cells did not break down the hydrogen peroxide, they would be poisoned and die. You will be using chicken or beef liver. Add 2 ml of hydrogen peroxide. Result lead to the fact that cold effect the rate of reaction in liver enzyme less than heat does. Record this amount on a table. Why doesn't boiled liver react with Hydrogen Peroxide like raw liver does? The hydrogen peroxide is broken down, so that it cannot be dangerous anymore, into water (which is given off when you perspire) and into oxygen (which can be given off when you exhale). Replace the bung quickly so that no gas is lost. Cite this article as: William Anderson (Schoolworkhelper Editorial Team), "The Effect of Hydrogen Peroxide on Liver: Hypothesis, Apparatus, Method," in. Set up the apparatus as in the diagram. 6. The test tube with boiled liver had no results. Does Oil of Oregano raise the sugar in your blood? This photo shows when we took liver and put it on the bottom of a clean test tube, put a small amount of water over it and boiled it for five minutes. Hydrogen peroxide was used in this lab, it was decomposed at the molecular level by the enzyme catalase showing the chemistry portion of the biochemistry. How do you remove the door panel on a Daewoo Matiz? Lesson organisation. In which test-tube is the enzyme inactive ... hydrogen peroxide mixture of boiled catalase and hydrogen peroxide mixture of catalase and boiled hydrogen peroxide 8 Four test-tubes are set up as shown. Record the reaction rate. Therefore my hypothesis is that the rate of decomposition will be at its fastest with a temperature of forty degrees Celsius and a surface area of grinded liver. Cells make the enzyme catalase to remove hydrogen peroxide. Add 2 ml of hydrogen peroxide. Replace the bung quickly so that no gas is lost. The test tube with iced liver had a lot of little bubbles and the liver began to float. The force of the reaction, described by a chemical equation H2O2 = 2 H2O + O2, causes the rapid creation of oxygen bubbles and foam on the liver surface. If you crush a small bit of the liver and pour it into a tube of hydrogen peroxide, then the reaction will take place much faster than if the liver was in room temperature. Record the reaction rate. Obtain a piece of liver that has been boiled for 5 minutes. When did organ music become associated with baseball? Repeat steps 5 & 6 with the boiled and frozen potato sections. Meaning that there was little to no reaction at all. Nothing is going to happen when you add H2O2 to the boiled potato because you have denatured the enzyme (catalase). Perboric acid decomposed 3.0 or 0.10 per cent per day. When you have finished, discard potato and liver in the trash (not the sink!) This is because there are more liver particles that will be exposed to the hydrogen peroxide. When the liver was boiled, the catalase enzymes were denatured and therefore unable to break down the hydrogen peroxide. Boiling of the liver tissue will denature the enzyme catalase and so the tissue will not be able to hydrolyze H2O2 into O2 and H2O. Fresh and boiled liver will catalyze the decomposition of hyrdogen peroxide. (1.4g), test tube (with delivery tube), beaker, Bunsen burner, thermometer, tripod, gauze, clamp and boss, stand. It is used as an oxidizer, bleaching agent, and antiseptic. To test the effect of a base, put one teaspoon of the blended liver in a bowl and add one teaspoon of baking soda. All Rights Reserved. Answer Save. Without them the reactions would be so slow that life would grind to a halt. If you heat liver in water to 37C, then catalase will be able to work at its best, because around 37C is the optimum temperature for many enzymes in living bodies. 1. The bubbling reaction you see is the metabolic process of decomposition, described earlier. CAUTION: Use a test-tube holder for hot test tubes. The reaction rate for the boiled liver and peroxide was zero. Hydrogen Peroxide Rare Liver Ice Hot Plate Multiple Beakers Independent Variable: The Independent Variables for this experiment are whether the Liver is put in with Hydrogen Peroxide, whether it is pre-heated or pre-cooled. Our liver is an organ that produces an enzyme called Catalayse whose purpose is to break down Hydrogen Peroxide (a toxin). 1. Place this test tube in a boiling water bath for 5 minutes. Catalyse is a base, and Hydrogen Peroxide is an acid. Hydrogen peroxide is harmful and must be removed as soon as it is produced in the cell. A larger amount of catalase lowers the activation energy, therefore speeds up the rate of reaction. Put equal quantities of liver into 2 clean test tubes and 1 ml H2O2 into 2 other test tubes. The point of this part of the experiment was to see how temperature would effect the enzyme catalase. Put one test tube of liver and one of H2O2 into an ice bath. Add 2 ml of hydrogen peroxide. Four test tubes, manganese dioxide (MnO 2), water, beaker, spirit lamp, piece of fresh liver or potato, hydrogen peroxide, boiled and cooled piece of liver or potato, boiled and cooled manganese dioxide solution.. Hydrogen peroxide is added using the measuring cylinder. Enzyme Preparations-All the experiments in this paper were carried out with highly purified rat liver catalase, prepared as described in Paper II (9). In this lab, you will study the catalase found in liver cells. When you ground the liver up, the surface area dramatically increases. 6. After 10 seconds record the amount of water displaced in the burette. 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